In the Report “PML Regulates Apoptosis at Endoplasmic Reticulum by Modulating Calcium Release,” there is a duplicated image shown in the last panel of Fig. 3D; the same field of cells is shown to represent cells treated with etoposide (ETO) in the presence and absence of the erPML chimera.
To determine whether the effects of Pml (promyelocytic leukemia gene) on the regulation of calcium-dependent cell death depended on its localization to the endoplasmic reticulum (ER), the authors generated a chimeric protein targeted to the outer surface of the ER (erPML). The introduction of erPML in Pml−/− mouse embryonic fibroblasts (MEFs) restored sensitivity to apoptosis induced by ER stress but did not restore the sensitivity to ETO (fig. S7A).
Figure 3D was intended to show representative microscopic fields of Pml−/− MEFs and Pml−/− expressing erPML before and after treatment with different apoptotic stimuli. However, these images were not used for the statistical analysis, which analyzed flow cytometry data reported in fig. S7A. The corrected Fig. 3D (below) shows images from a newer experiment that confirmed the original results.
The error in Fig. 3D does not undermine the work’s conclusion. The authors have repeated the experiments and obtained identical results.